Rainin Pipettes are handheld instruments used in pharmaceutical, biotech and R&D labs to transfer measured amounts of liquid. METTLER TOLEDO offers manual and electronic single channel and multichannel pipettes for all your liquid handling needs along with preventive maintenance, calibration and equipment repair services.
Pipetting accuracy depends on the interplay between the pipette, the tip and the person using it. Check out our Good Pipetting Practice Online Seminar to learn more.
RAININ XLS combines cutting-edge engineering and innovation to deliver the most comfortable, accurate and precise manual pipettes. Designed for use with either hand, their sure-fit handles and light springs with “stiction-less” pipette-tip sealing technology offer effortless operation and reduced risk of RSI. Up to 35% lighter than traditional multichannel pipettes, XLS+ models require minimal arm effort and are easy on your hands, even after hours of pipetting. XLS+ pipettes are also up to 30% faster to adjust and deliver results, thanks to a new, innovative push-bar design.
Exceptional Precision, Accuracy and Reliability
XLS single-channel and XLS+ 8 and 12 channel manual pipettes feature a new liquid end with durable materials and a mechanical design delivering exceptional accuracy and precision. They also deliver industry-leading channel-to-channel consistency, thanks to a proprietary push-bar design that allows for more consistent tip mounting and sample pickup.
Redesigned Liquid End for Smooth Autoclaving
Developed for high-throughput labs, XLS+ single-channel and multichannel pipettes have a fully redesigned liquid end with elastomeric seals and polymer tip ejectors for fast, easy cleaning and autoclaving. The new elastomeric seals and nozzles are engineered for smooth, low-force ejection of tips with no rocking or hand tightening required. XLS+ pipettes are also 35% lighter and require less arm effort than traditional pipettes, making them easy on your arms, hands and wrists.
A Snag-Proof Volume Lock for Enhanced Ergonomics
The new snag-proof volume lock on the XLS+ series is easy to modify and secure, even when wearing gloves. It also prevents accidental volume drift for more precise performance and ensures your work is always delivered with the same precision.
RFID Tag for Calibration Tracking
Featuring RAININ’s innovative RFID tags, the XLS+ series is the first pipette to allow you to easily record and retrieve calibration data and service records on-board. This new feature saves time and improves compliance in regulatory controlled laboratories.
Pipettes are an essential tool in almost every life science laboratory. Whether you’re doing DNA cloning, cell culture, or other microplate-based applications, you need to be able to transfer and dispense accurate volumes of samples. The Pipet-Lite XLS is designed to do exactly that. This manual multichannel pipette features ergonomics that reduce hand fatigue and discomfort while maintaining accuracy and precision. This is made possible by a combination of a light spring design, new polymer tip ejectors and Rainin’s LiteTouch System technology. This results in a plunger stroke that is smooth and precise, yet requires less force than ever.
This model also features state-of-the-art design that delivers supreme accuracy and precision, as well as a low cost of ownership. An embedded RFID tag allows for advanced asset management, including GLP user logging and service alarms. It is password protected to prevent unauthorized modifications of settings, protocols and other parameters. It works with LTS tips and is fully tamper proof. It also comes with a rapid charging station and can automatically go into sleep mode after 10 minutes of inactivity to conserve battery power.
Its redesigned ergonomic handle rests perfectly in your hand reducing tiredness and compromised pipetting function. The finger hook facilitates a comfortable grip while the high-quality PVDF material helps to limit transfer of hand heat to the inside of the pipette. The snag-proof lock design also prevents accidental volume drift and is easy to adjust even when wearing gloves.
A manual multichannel pipette is a handheld life science research instrument that can transfer or measure microliter volumes of liquids across multiple plates, tubes or other sample containers. These manual devices are typically used to transfer cells, reagents or other samples for use in experiments. They offer the advantages of being lightweight, easy to use and highly repeatable. Depending on your application, some models can be equipped with additional features such as multi-dispense, mixing, reverse pipetting or an automated calibration feature.
DiamondLink PCR Tubes & Caps
PCR tubes and caps play an important role in ensuring accurate and reproducible results. They are in direct contact with reagents and samples and require special material properties, which can withstand temperature changes during thermal cycling, prevent condensation, provide sample protection and impart leak-proof sealing. They must also be easy to handle and offer good optical transmission. Moreover, they must be produced in a clean production workshop and undergo strict QC procedures. The Diamond PCR Tubes and Cap Strips from Eppendorf offer all of these benefits and more.
They are made from medical grade polypropylene, which is biodegradable and resistant to a wide range of chemicals. These features make them ideal for use in PCR, real-time (RT) and qPCR experiments. Moreover, their thin walls enable efficient heat transfer and reduce cycle time. They are also RNase, DNase, Human Genomic DNA, and PCR inhibitor free. Additionally, they are suitable for use with most thermal cyclers, sequencers, fragment analysers, and genotypers.
The PCR tubes have attached caps that are easy to open and close without tools, providing optimal seal to minimize evaporation. They are available with domed caps, flat caps, and optically clear strip caps. These caps can be easily labeled using a pen or barcode stickers. Moreover, they are easily identifiable due to the alphabet letters on the hinge of the cap.
Additionally, the PCR tubes have a low-profile design that ensures minimal dead space between the caps and heated lid of the thermal cycler, preventing condensation and reducing sample loss. This ensures better and more reliable results. Additionally, the PCR tubes are easy to grip and have a smooth surface, which makes them less likely to scratch or deform during handling.
To minimize potential contamination, PCR tubes should be sealed in their original packaging until they are ready for use. They should be organised on a clean 0.2 mL tube rack and labeled clearly with a marker. Afterwards, they can be stored in a clean 0.2 mL tube box or in a clearly labelled ziplock bag.
NEST PCR Tubes
PCR is an essential tool for the diagnosis of fungal diseases, as well as for determining the presence of bacterial and viral pathogens in plants. Conventional bench-top PCR systems require complex machinery, expensive reagents and trained personnel to operate. They are also difficult to bring on-field, due to their requirement of gel electrophoresis in a laboratory setting to quantify and identify the amplified DNA.
Multiplex PCR platforms allow the simultaneous detection of target sequences in a single reaction. This method is useful in reducing the number of reactions and reagents needed, as well as cutting down on sample preparation time and overall testing costs. These platforms can be used to detect a wide range of diseases and infections, including community-acquired respiratory infections such as influenza, mycoplasma and Chlamydophila; agents of gastrointestinal infection such as Campylobacter, Salmonella, Shigella, Clostridioides and Giardia; and causative agents of meningoencephalitis including Neisseria meningitidis and herpes simplex virus.
One challenge of multi-tube PCR is the contamination of the second round of amplification by off-target sequences generated by the first reaction. This is addressed by the use of nested PCR, which involves the use of two pairs of primers. The first pair binds to the flanking regions of the desired sequence, while the second pair anneals to sites within the sequence itself. This ensures that only the desired sequence is re-amplified in the second reaction, eliminating off-target sequencing products and increasing assay sensitivity.
A disadvantage of nested PCR is the need to transfer the external-primer product from the first-round reaction to the second-round internal-primer reaction, which can introduce carryover contamination and make it more difficult to quantitate the resulting amplicons. This can be overcome by physically separating the first-round and second-round reactions using a layer of wax or oil, but this can still result in contamination in the form of a non-specific amplicon.
Another way to reduce carryover is by using a molecular beacon probe, which consists of a fluorescent reporter dye and a quencher molecule that form a hairpin structure in the target DNA. The quencher molecule blocks the fluorescence of the reporter dye, which can only be detected when it is cleaved by a corresponding binding site in the PCR product.